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For selectivity studies, recombinant GSTO1 (1 M), PDI (1 M), BSA (1 M) or GRP78 (1 M) in reaction buffer were incubated with C1-27 or DMSO for 30 min at 37 C, followed by incubation with CMFDA (500 nM), BODIPY- C1-27A (1 M) or DMSO for 30 min

This is done by a complicated, advanced pharmaceutical method

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This unique composition endows KT-474 with its selectivity in targeting IRAK4 for degradation, which is a significant feature in the realm of PROTAC-based molecular regulation