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Determination of intracellular reactive oxygen species (ROS) levels Total intracellular ROS levels were determined using a ROS assay kit (cat

Toxicant channeling A hypothesis in which it is suggested that reactive sulfur-containing species generated from toxic cysteine S -conjugates are channeled from the active site of mitochondrial aspartate aminotransferase to -ketoglutarate dehydrogenase complex, thereby accounting for the remarkable sensitivity of this enzyme complex to inactivation in tissues/cells exposed to certain toxic cysteine S -conjugates

The protein was purified with Strep-affinity chromatography (for more details see supplementary figure 2), the tag was removed by TEV-cleavage and the protein was dialyzed into buffer W (100 mM Tris-HCl, 150 mM NaCl, 1 mM EDTA, pH 8.0)

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doi: 10.1055/s-2007-978596

and for vial-size principles generally, see Peptide Vial Sizes Explained