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These mutations lead to mitochondrial dysfunction and impaired energy production, resulting in the death of retinal ganglion cells and optic nerve damage

Briefly, cells are cultured with the STEMdiff TM definitive endoderm basal medium with supplements A and B for 24h followed by STEMdiff TM definitive endoderm basal medium with supplement B for 48h with daily medium change ( TM DE kit is based primarily on human activin A and Wnt3a ( Hepatoblast specification For small molecule hepatoblast specification, DE cells were washed with DPBS / and knockout DMEM (Thermo Fischer Scientific) and sequentially cultured with hepatoblast specification medium without passaging

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