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2000 Oct;20(19):7311-8

Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark

The particular example shown here is a schematic diagram of a method for evolving a genetic vaccine vector for improved oral delivery

also confirmed that inhibition of oxidative stress can enhance collagen fibers, changes in trabecular bone density, and improve changes in bone microenvironment (73)