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After two freeze-thaw cycles, 20 L of 5% 5-sulfosalicylic acid (SSA) was added, followed by centrifugation at 8,000g for 10 min

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Because limbic structures have significant bidirectional connections with the hypothalamus and autonomic bed nucleus of the stria terminalis, one might speculate that limbic dysregulation may contribute to the often-noted autonomic and neuroendocrine dysregulation in bipolar patients (125)

While clinical trials on the use of the combination of compounds as treatment for diabetes in humans have begun in other jurisdictions such as India, Jamaica currently has no such programme