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To isolate the barcode and IS, we allowed 3 mismatches in the 23 bp invariant sequence upstream and up to 6 mismatches in the 50 bp downstream of the barcode using a Hamming distance function to compare the expected and actual sequences

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Yes, to some extent this is similar to how a specific J1939 parameter group (PG) will contain multiple SPNs/signals (aka data parameters) in the 8 data bytes

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of this final rule with comment period, CMS is finalizing, that beginning January 1, 2026, if a hospital's payer specific negotiated amount is based on an algorithm or percentage, the hospital would be required to describe the percentage or algorithm and report a new data element, the median allowed amount, instead of the estimated allowed amount, and that the median allowed amount is defined as the median of the total allowed amounts the hospital has historically received from a third-party payer for an item or service for a time period no less than 12 months and no longer than 15 months prior to the date the MRF is posted