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Cells were then selected and maintained in culture media containing 1 g/mL of puromycin

These data indicate that the S-crystallins consist of a family of enzymatically inactive proteins (when using CDNB as a substrate) which is considerably larger than previously believed and that GST activity was lost by gradual drift in sequence as well as by insertion of an extra peptide by exon shuffling

The S6K protein family in health and disease

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