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Briefly, mice bearing TSC2 -/- ELT3 xenograft tumors were treated every other day for 5 days with vehicle or rapamycin (1 mg/kg on day 1, 3, and 5) and tumors were harvested for metabolite extraction, as above, 3 h after the final treatment

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The 3- and 5-Gy groups had significantly shorter survival than the 0-Gy group (Supplementary Fig

Confocal Microscopy and Spectral Analysis Channel mode confocal images of BODIPY, Nile Red, and GFP fluorescence in late stage L4 animals were acquired essentially the same as previously described [21]