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The following day, plates were washed four times with 1x PBS and 0.02% Tween 20 (ELISA wash buffer) prior to blocking for 1 h with 90 l/well of PBS containing 10% non-fat milk and 0.02% Tween 20 (blocking buffer)

SIRT5 expression in lung cancer tissues positively correlates with GLUD1 activity, and patients with high SIRT5 expression exhibit larger tumors

3.5.4 Metabolic system Pro-inflammatory cytokines also impair insulin signaling, leading to hyperglycemia and type 2 diabetes (124)

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NCT04847557)