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Modification of primary amines to higher order amines reduces in vivo hematological and immunotoxicity of cationic nanocarriers through TLR4 and complement pathways

Middeldorp JM Functional Delivery of Viral miRNAs via Exosomes

Caspase 3 activity assay Fifty milligrams of frozen remnants livers were lysed in caspase 3 activity buffer (20 mM PIPES, 100 mM NaCl, 10 mM DTT, 1 mM EDTA, 0.1% CHAPS, 10% sucrose, pH 7.2)

doi: 10.1002/acr.21660 42 ParksCGHoppinJADe RoosAJCostenbaderKHAlavanjaMCSandlerDP

Indeed, HA-tagged NEDD8 or ubiquitin, but not SUMO2 or SUMO3, immunoprecipitated modified high-molecular weight IL-1 when expressed in 293T cells (Supplementary Fig

MDA was evaluated by measuring the thiobarbituric acid reactive species (TBARS)