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MicroRNA1917 targets CTR4 splice variants to regulate ethylene responses in tomato

doi: 10.1021/jacs.6b03344 135 KitadaMKoyaD

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Following the treatments, cells were washed with ice cold PBS and lysed in ice cold RIPA buffer (50 mM Tris HCl pH 8, 150 mM NaCl, 0.5% sodium deoxycholate, 1% Nonidet P-40, 0.1% SDS, 1 mM EDTA) containing 1 mM phenylmethanesulfonyl fluoride (PMSF) and 1 protease and phosphatase inhibitor cocktail (Sigma-Aldrich Chemie GmbH, Munich, Germany)