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Reproduct Dev Med

We found that both mRNA and protein levels of Gclc were significantly higher in B6 MZB compared to B6 FoB (Fig

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Therefore, the differences in these proteins could explain the reduced expression of GshF in the mutant ZJ12345 compared to the wild type strain and help explain why the mutant required a higher IPTG concentration to obtain GshF activity similar to the wild type host strain