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10.1080/13880209.2022.2096644 46 LuanZ.FanX.SongH.LiR.ZhangW.ZhangJ

whether there is a rate-limiting pathway or synergistic/antagonistic crosstalk across cell types and pathological conditions has not been systematically evaluated

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Specificity of GST-GFP to GSH laden hydrogel homogenates To confirm the specificity of the interaction, nickel-purified GST-GFP was incubated in the presences of homogenates made with equimolar GSH (Figure 3A, top) or cysteine (Figure 3A, bottom)

Immunoproteasome activity 20 g of liver samples from ibuprofen-treated and control animals were incubated with immunoproteasome buffer (50 mM Tris, 5 mM MgCl 2 , 20 mM potassium chloride (KCl), 1 mM DTT (freshly added), pH 7.5), and inhibitor or an equal volume of dimethyl sulfoxide (DMSO) for 20 min at room temperature

When used together, the response is generally more consistent and more complete