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Group 2: Preg-30 group (n = 8), mice were injected with pregabalin (30 mg/kg, i.p.x 4) and vehicle for eight days during the acquisition phase

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Mature Sertoli cells were enriched in Sertoli cell development, male gonad development, BMP signaling pathway, SMAD protein signal transduction, cellular response to corticotropin-releasing hormone stimulus and some GO terms related to development and proliferation (Fig