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doi: 10.1371/journal.pone.0194679

The expression of the indicated proteins was shown by immunoblotting

Studies for which no response was received were excluded

Primary cultures of satellite cells were prepared from the upper hindlimb and back muscles of 910 month-old male rats and cultured for 24 h beginning at 24-h post-plating in DMEM-10% HS containing 5 ng/ml HGF that was pre-treated with GSSSG and LASSS separately at 1:4000 molar ratio just prior to peroxynitrite treatment (1:2000 to HGF

We try to limit the number of injection points we use, and we use cannulas like this technique does (because it helps limit swelling and bruising), but we dont limit ourselves to a single injection point for everyone