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The N-terminal tryptophan and three glycine residues give the molecule a flexible backbone that has not, in the published literature, been shown to adopt a stable secondary structure under physiological conditions

For comparison, our IGF-1 LR3 research provides insight into how IGF-1 signalling is studied as a separate downstream variable

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2.1.2.2 Enzymatic ligation: Sortase Amediated covalent precision Sortase Amediated transpeptidation offers a site-specific covalent bond between an LPETG-tagged antigen and an N-terminal oligoglycine motif on the VLP surface (Figure 1C