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To confirm that the overexpression of HRASV12 and cyclin E1 via retroviral transduction induced R-loop formation, we isolated genomic DNA from U2OS cells transduced with the respective retroviral vector and performed slot blot analysis using the S9.6 antibody, which recognizes RNA:DNA hybrids

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After centrifuging the plates for 5 minutes at 1,500 rpm, duplicate wells were harvested and lysed with 350 l of RLT buffer

The Initial Coverage Limit no longer exists in 2025 and beyond

Less than four months later, the Richmond newspapers reported that George and his brother Ernest, age 18, had been captured in their attempt to cross to the Union army lines and were imprisoned at Castle Thunder, a notorious jail