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Furthermore, it is interesting to note that, with the exception of abcZ , which encodes an ABC transporter, the other six housekeeping genes used to analyze the structure of the meningococcal population by MLST to identify strains belonging to certain ccs, more or less associated with meningococcal disease or carrier status, encode enzymes involved in the main metabolic pathways (Yazdankhah et al., 2004

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Embryos were deteriorated by forceps, anesthetized in tricaine methanesulfonate solution (Sigma-Aldrich), mounted in 1% methyl cellulose on a depression slide (Aquatic Eco-Systems, Apopka, FL, USA) and photographed under the stereomicroscope Z16 (Leica Microsystems, Ernst-Leitz-Strasse, Germany) for observation

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