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Androgen is mainly secreted by the testis, androgen deficiency will lead to a series of pathophysiological conditions, which will damage the sexual function and overall health of the body ( In clinic, androgen replacement therapy can restore the serum testosterone level to normal, and improve the sexual desire of patients with hypogonadism

Claudin-5 is a major cell adhesion molecule that forms a paracellular barrier between ECs, induced an increase in BBB permeability with the absence of claudin-5 (Menard et al., 2017

Distinct from the closed (post ATP hydrolysis) NaAtm1 structure, a pocket is present, although relatively small, which however is empty as also observed in the occluded NaAtm1 states and for ScAtm1 and AtAtm3 (Fig