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Brown adipose tissue (BAT), brain (cortex), heart, intestine, kidney, liver, lung, muscle, spleen and white adipose tissue (WAT) were dissected and quickly frozen in liquid nitrogen immediately and stored at 80 C until metabolite extraction

Adv Mater 32(33):e2003563

The membrane was baked for 2 h at 80 C and then blocked in 5% milk that was diluted in a PBS buffer for 1 h at room temperature

Upon ER stress, they are transported to the Golgi where they are proteolytically cleaved and then translocate to the nucleus, where they induce UPR genes

NSAID-induced inhibition of the COX pathway leads to shunting of AA metabolism down the 5-LO arm (Palikhe et al., 2009

A high-resolution in vivo atlas of the human brains serotonin system