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Adherent glial cells were washed in PBS and incubated at 37C with 1:5 dilution of 10 Trypsin-EDTA (Sigma-Aldrich) in high glucose DMEM until astrocyte detachment was complete (25 min)

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In preclinical research models, it has been studied extensively for its involvement in angiogenesis (new blood vessel formation), fibroblast activity and collagen synthesis, growth factor modulation (including VEGF and EGF pathways), connective tissue signaling in muscle, tendon, and ligament models, gastrointestinal mucosal integrity, and inflammatory cytokine modulation

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It promotes collagen synthesis, blood flow, and cellular repair helping the body naturally regenerate after injuries or surgery

Immunohistochemical localization and quantification of glucose transporters in the mouse brain