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(PubMed) Iyer R, Gupta A, Khan A, Hiremath S, Lokhandwala Y

Among 17 members in humans and 16 members in mice, PARP1 alone is responsible for about 90% of all NAD + used by the PARP protein family [49]

The release of fluorogenic AMC from the substrate was measured by Infinite M200 fluorometer (Tecan, Mnnedorf, Switzerland) at 24 C in a 30 min kinetic cycle (2-min intervals)