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5.2 Analytical Platforms and Quality Control for GSH/GSSG Measurement Once sample integrity has been protected, the analytical platform determines selectivity, sensitivity, throughput, and whether GSH and GSSG are measured directly

The experiments with isolated mitochondria (0.5 mg/mL) were carried out at 28C with a standard reaction medium containing 15 M Ca 2+ , 10 mM Hepes, 125 mM sucrose, 2 mM K 2 HPO 4 , 65 mM KCl, 1 mM MgCl 2 , and 5 mM glutamate plus 5 mM malate (pH 7.2)

The calculator updates instantly as you change inputs, displaying the concentration in a prominent result card

Usually more than one treatment will be recommended

Baird, P

RABGAP1 KD cells were lifted with Accutase and plated at 1 10 6 cells/well in a six-well plate in 2 ml E8 supplemented with ROCKI