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The protocol comprised eight stressors: food deprivation for 24 h, water deprivation for 24 h, flash stimulation (150 flashes/min for 5 min), cage tilting at 45 for 24 h, overnight illumination for 8 h, wet cage environment (200 mL water added to sawdust bedding) for 24 h, tail suspension for 5 min, 60 C heat stimulation for 6 min, and nipped tail for 5 min

Nature (2002) 419(6904):31621

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All TaqMan primers were designed for amplifying exon-exon junction

Infection is often asymptomatic

S., Outridge, P