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The lower chamber was added with various materials supplemented with 10% FBS, while serum-free DMEM was added to the upper chamber

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Notably, RSL3 induced widespread depletion of the astrocyte-like signature in transformed glioma cells across patient samples, including several genes with important metabolic functions, such as FABP7 (fatty acid binding protein 7), involved in fatty acid and mitochondrial metabolism, DBI (Diazepam Binding Inhibitor, Acyl-CoA Binding Protein) involved in lipid metabolism, and GPM6B (glycoprotein M6B), membrane proteolipid with important roles in membrane trafficking (Fig

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