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A 15-nt elongation product is detected with the 3 vRNA-1 template (lane 2, orange arrow), while the control reaction yielded a 9-nt product (lane 1, red arrow)

Finally, in the plant-rhizobium symbiosis, nitrogenase is protected by plant-produced leghemoglobin, a heme-containing O 2 scavenger that buffers O 2 to levels sufficient to support respiration, but not high enough to damage nitrogenase (Rutten & Poole, 2019)

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HPLC analysis confirms the purity of the peptide, mass spectrometry (MS) verifies the molecular identity, and additional ICP or AAS analysis determines the copper content in the complex