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For haematoxylin and eosin (HE) staining, the samples were stained with haematoxylin solution (FUJIFILM Wako Chemicals Corporation, Osaka, Japan) for 5 min, washed with running water for 10 min, and then stained with eosin solution (FUJIFILM Wako Chemicals Corporation) for 3 min

J Clin Pathol 54(3):176186

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Traditional biomarkers are usually insufficient in the early stages of this process