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Yet another aspect of the invention is to provide a method for treating patients not responsive to a PDE-5 inhibitor alone, the method including administering both PT-141 and a PDE-5 inhibitor, wherein the PDE-5 inhibitor is administered at approximately the same time as administration of PT-141

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term is 114 days) and showed that the blue staining following treatment with slightly acidic solutions of iron salts (Prussian blue reaction) was confined to the neural tube and did not penetrate out into rest of the embryo