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In patients with T2DM who do not have atherosclerotic cardiovascular disease (ASCVD)/indicators of high-risk, HF, or CKD and who need to minimize hypoglycemia and/or promote weight loss, GLP-1 RAs are generally recommended as a second or third-line option as add-on to metformin therapy

Tissue samples were snap frozen and homogenized in Lysis Buffer with protease inhibitor using Tissue Lyser (Biorad) for 3 min

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For E cr -normalized data, moderate and severe proteinuria were defined as E pro /E cr 100 mg/g creatinine and 150 mg/g creatinine, respectively [19]

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Acute nicotinamide riboside supplementation increases human cerebral NAD+ levels in vivo