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Presenting as macules/papules on the palms and palmar aspects of fingers, these lesions are often painful and cause significant morbidity (Figure 5B)

2.14 In vitro angiogenesis (tube formation) assay The in vitro angiogenic activity of HUVECs (human umbilical vein endothelial cells) was determined by the Matrigel tube formation assay

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To assess whether Cas12f delivery via EVs requires MIM, we attenuated protrusion formation by introducing a five-lysine-to-alanine (5KA) mutation in the MIM I-BAR domain 54 , which was previously shown to impair EV generation 40

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