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T cell-intrinsic IRF5 regulates T cell signaling, migration, and differentiation and promotes intestinal inflammation

The peptide crosslinker, GKK C GPQG IWGQ C KKG was synthesized using standard solid-phase peptide synthesis on FMOC-Gly-Wang resin (EMD) on a Liberty 1 Microwave-Assisted Peptide Synthesizer (CEM) with UV monitoring, as previously described 4,5,37

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Briefly, plasmid-cured EcN was transformed with pSIM18 containing the exo, bet , and gam genes expressed from the pL operon under the control of a temperature-sensitive (ts) repressor, CI857, and a hygromycin resistance cassette 89

Metabolite extraction was performed with ethanol and then centrifuged to extract the supernatant