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The importance of NO as a mediator of skin inflammation is underscored by the delicate balance between its production and degradation, which is closely related to the onset and development of inflammatory skin diseases

harvested cells were first re-suspended in chilled lysis buffer (20 mM Tris [pH 7.9], 0.5 M NaCl, 5 mM imidazole, 10% glycerol, 1 mM DTT, 1 mM PMSF) and the lysis mixture was sonicated on ice

Beigel F, Steinborn A, Schnitzler F, Tillack C, Breiteneicher S, John JM et al

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In this paper, structome analysis of non pathogenic Mycolicibacterium smegmatis , basonym Mycobacterium smegmatis , was performed