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and 5,789,215 to Berns et al., and U.S

Biochemical assays After fasting for 4 h, blood was collected to extract serum, and serum TC and TG concentrations were measured using kits provided by Nanjing Jiancheng Bioengineering Institute, Nanjing, China

The dry matter (DM) and organic matter (OM) were determined for the diets according to Sample Collection Blood Collection and Analyses On d 0, d 35, and d 65 after overnight fasting, 3 mL of blood samples was collected from each kitten from the forelimb vein, blood was transferred to a pre-cooled serum separator tube, and left to stand for 30 min before centrifugation at 3,500 g at room temperature for 15 min

We tested polarized and non-polarized macrophages, under stimulated and unstimulated conditions, using various differentiation protocols and treatment regimens

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