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Data analysis The SPSS (Ver

Box 1 Clinical Practice Guidelines for Vitamin D 3 Immunomodulatory Applications Therapeutic Targets: Optimal Range: Maintain serum 25(OH)D levels between 40-60 ng/mL for maximal immunomodulatory benefits (guideline) ( Minimum Threshold: Ensure levels exceed 30 ng/mL for basic immune function support (guideline) ( Safety Ceiling: Avoid exceeding 100 ng/mL to prevent hypercalcemia and adverse effects (guideline) ( Dosing Guidelines: General Population: 15002000 IU Vitamin D 3 daily for adults with deficiency or insufficiency (guideline and consensus statement) (66)

Clinical presentation varies significantly, ranging from clinically silent macrocytosis to life-threatening anemia or pancytopenia

42:51-55 (2004) Engel SR, Dietrich FS, Fisk DG, Binkley G, Balakrishnan R, Costanzo MC, Dwight SS, Hitz BC, Karra K, Nash RS, Weng S, Wong ED, Lloyd P, Skrzypek MS, Miyasato SR, Simison M, Cherry JM

For transfection, siRNAs were delivered to a final concentration of 10 nM using Lipofectamine RNAiMAX transfection reagent (ThermoFisher Scientific) as detailed by the manufacturer

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