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The absorbance was then measured at 417 nm using a 96-well ELISA microplate reader (VersaMax, Molecular Devices, San Jose, CA, United States)

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Avoid exposure to sunlight and heat

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The 5-O-CH3 group can form a single hydrogen bond with the adjacent 5-phosphate group, thereby stabilizing the local ribose-phosphate backbone conformation and potentially facilitating base-pair recognition during protein synthesis [121]