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Harada K, Wang X, Shimodaira Y, Sagebiel T, Bhutani MS, Lee JH, Weston B, Elimova E, Lin Q, Amlashi FG, Mizrak Kaya D, Lopez A, Blum Murphy MA, Roth JA, Swisher SG , Skinner HD, Hofstetter WL, Rogers JE, Thomas I, Maru DM, Komaki R, Walsh G, Ajani JA

Cell pellets were collected and lysed in 9 M urea or RIPA buffer (10 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 0.5% Na deoxycholate, 0.1% SDS, and 1% Triton X)

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Second, multimodal therapies combining urate-lowering agents, anti-inflammatory cytokines, and immunomodulators within a single platform may synergize to halt disease progression while minimizing systemic side effects