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Nevertheless, the association between low levels of ROS and the generation of double-strand breaks remains a topic of debate, partly owing to methodological challenges, such as those encountered with the neutral Comet assay and the use of nonspecific biomarkers for double-strand breaks, such as gamma-H2AX, an early cellular response to the induction of DNA double-strand breaks

doi: 10.1177/000313481007601226 5 AntoniouACEastonDF

ME/CFS is a remarkably misunderstood disease, and many patients have experienced judgement, prejudice, and disbelief from healthcare professionals (39) as some refuse to view ME/CFS as an indisputable clinical entity (39) and question its legitimacy (40, 58) delays any possible early diagnosis for the patients and has also resulted in psychologisation of the disease (40, 53)

In fact, many adults are completely unable to absorb B-12

Given that CSCs exhibit lower ROS compared to their non-tumorigenic counterparts [36,37,38], we treated cells with ROS scavenger NAC and found that it could counteract the Hedgehog signaling alterations induced by GPX2 inhibition

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