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AtGLXI.1 and AtGLXI.2 display a higher activity with Ni 2+ using MG and GSH as substrates whereas Mn 2+ is preferred for the conversion of GO and GSH

Then, the solution was stirred for 1 h

Antibodies: Affinity-purified, HRP-conjugated, rabbit anti-GST antibody 18844P used for WB at 0.2 g/ml (1:5,000)

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The roles and functions of various prebiotics commonly used in aquaculture are summarized in Table 2 , providing insights into their sources and specific benefits for fish health and immunity