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Reverse transcription and qPCR reactions were performed as described by III Reverse Transcriptase (Invitrogen) and Oligo (dT) 20 Primer (Invitrogen)

Yes Are the conclusions drawn appropriate in the context of the current research literature

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Ann N Y Acad Sci 939:162178 Vogelzangs N, Beekman AT, van Reedt Dortland AK, Schoevers RA, Giltay EJ, de Jonge P, Penninx BW (2014) Inflammatory and metabolic dysregulation and the 2-year course of depressive disorders in antidepressant users

The interconnections between proteins and their functions were visually represented using a Circos plot, created with the BioLadder online analysis software ( Western blot Sciatic nerve tissues were homogenized using an electric tissue homogenizer (Fluka, Inc.) with a 10 s on/10 sec off cycle on ice