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S100A7 lacks the ability of Ca 2+ -binding at the amino acid EF-hand motif, due to the absence of three amino acid residues and non-conserved substitution at position 12 of the EF-hand loop region (69)

Purified rat acid-labile subunit and recombinant human insulin-like growth factor (IGF)-binding protein-3 can form a 150-kilodalton binary complex in vitro in the absence of IGFs

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Neuropsychiatric symptoms may precede the blood signs entirely, presenting as myelopathy, neuropathy, dementia, and less often optic nerve atrophy