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Materials and methods Animals All methods are reported in the paper in accordance with ARRIVE guidelines ( The animals used in this study were Wistar rats originating from the husbandry of the Department of Experimental Neuropathology of the Institute of Zoology and Biomedical Research, Jagiellonian University in Krakow

The heavier red blood cells sink to the bottom of the testing tube while the platelet-rich plasma and platelet-poor plasma remain at the upper part of the testing tube

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To maintain long-term cellular health and protect against oxidative stress, a maintenance session once every 4 to 6 weeks is often recommended

Studies document NO production effects on endothelial proliferation and vessel dilation

ACE2: Angiotensin converting enzyme 2, NK: Natural killer Cells, MIP1-: Macrophage inflammatory protein 1 , MIP1-: Macrophage inflammatory protein 1 , MCP1: Monocyte chemoattractant protein 1