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Glutamine is first transported into the cell and then into the inner mitochondrial membrane via specific transporters before being converted into glutamate by mitochondrial glutaminase

Importantly, several studies describing anti-ferroptotic activity are based mainly on general reductions in ROS or oxidative stress markers, while ferroptosis-specific confirmation requires assessment of key indicators such as GPX4 regulation, glutathione depletion, lipid peroxidation products, and iron-dependent rescue experiments [94, 95]

Data demonstrated that the inhibitor ML385 alone did not significantly affect cell viability but significantly inhibited the protein expression of p-Nrf2 (Fig

6, E and F)

Dull or uneven body skin

Full-length DORN1 (At5g60300) or RBOHD (At5g47910), as well as sub-genic fragments DORN1 -KD (kinase domain) or RBOHD -N (N-terminal region, 11128 bp), were amplified using gene-specific primers (Supplementary Data 2) with cDNA derived from wild-type plants