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We start by exploring absorption of nitrate fertilizer and biosynthesis of alkaloids by tobacco plants, followed by the actions of bacterial nitrate reductases and/or chemical nitrosation occurring post-harvest, and end with human absorption from ST products followed by metabolism, adduct formation, DNA repair, and lastly the formation of mutations that lead to the progression of cancer (Section 4 select technologies or approaches that have been successfully used to limit or minimize the formation or introduction of TSNAs and other carcinogens during ST cultivation or production