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Cellular ROS detection assay After treatment with 0.5 mM glucose with or without 20 mM lactic acid, MCF-7 cells were washed with PBS twice, cultured with the medium with 25 2 DCFDA probe for 30 min, and the fluorescence intensity detected by excitation at 485 nm and emission at 535 nm

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The frequency should be set by the supervising clinician against a specific goal, not sold as an open-ended subscription