CRISPRa dCas9-VPH Expression Construct CRISPR sgRNA Libraries With around 69

With around 69

CRISPRtest_Blue™ Mouse Functional Cas9 Essential-Gene Knockout Assay for Murine Cells

The tet-inducible U6 promoter drives sgRNA expression and is responsive to the tetracycline analog doxocycline

The reagent kit contains everything necessary for library preparation—requiring no specialized equipment beyond a standard thermal cycler and an NGS platform

Assay primers contain Validator Barcodes (VBCs) to enable accurate quantitation of each clonotype

CRISPRa dCas9-VPH Expression Construct CRISPR sgRNA Libraries With around 69Lentiviral construct expressing the dCas9 VPH complex a dCas9 activator used with CRISPRa. Catalog # Description Vector Type SVVPHC9B PS CMV_dCas9_Blast CRISPRa dCas9 VPH pRDVCCB CMV dCas9 VPH 2A Blast plasmid SVVPHC9B VS CMV_dCas9_Blast CRISPRa dCas9 VPH pRDVCCB CMV dCas9 VPH 2A Blast virus SVVPHC9E2B PS EFS_dCas9_Blast CRISPRa dCas9 VPH pRDVCE2B EFS dCas9 VPH 2A Blast plasmid SVVPHC9E2B VS EFS_dCas9_Blast CRISPRa dCas9 VPH pRDVCE2B EFS dCas9 VPH 2A