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This updated burden estimate is a decrease of 960 hours [(3,200 HOPDs 0.167 hours 2 quarters)(3,200 HOPDs 20 percent 0.167 hours 1 quarter)] at a savings of $52,858 (960 hours $55.06) for the CY 2027 reporting period, and a decrease of 2,027 hours [(3,200 HOPDs 20 percent 0.167 hours 3 quarters) + (3,200 HOPDs 80 percent 0.167 hours 4 quarters)] at a savings of $111,607 (2,027 hours $55.06/hour) annually beginning with the CY 2028 reporting period

Thus, data coming from the bioreactor and also from external devices (gas analyser, substrate addition pump, and ethanol sensor) were integrated into Eve , which acted as a Supervisory Control And Data Acquisition or SCADA system (Brunner et al., 2021

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