sgOCT4 CRISPRa Controls Vector | Format – SELECT ONE::sgOCT4 Dual sgRNA in pRSL10Ble-mU6-sg1-hU6-sg2-UbiC-Tag RFP-2A-Bleo | Plasmid Unique Dual Index Set C

Unique Dual Index Set C for DriverMap EXP or AIR (193-288)

BFP) as well as a second construct with a non-target sgRNA and a second fluorescent marker (usually RFP or BFP)

Both the 43K and 87K libraries are designed using the criteria for effective guides described by DeWeirdt et al

and targets all pig protein-coding genes (~22

Lentivirus expressing luc with BlastR (virus)

sgOCT4 CRISPRa Controls Vector | Format<span class="variant-dropdown"> – SELECT ONE:</span>:sgOCT4 Dual sgRNA in pRSL10Ble-mU6-sg1-hU6-sg2-UbiC-Tag RFP-2A-Bleo | Plasmid Unique Dual Index Set CCellecta's sgOCT4 CRISPRa control constructs are human OCT4targeting sgRNAs in Cellectas standard CRISPRa lentiviral backbones, available either as plasmid DNA or packaged lentiviral particles for direct transduction. SinglesgRNA controls in pRSG16 U6 (sg) UbiC RFP Puro provide a straightforward positive control for CRISPRa activation, while the dualsgRNA pRSL10Ble mU6 sg1 hU6 sg2 UbiC TagRFP 2A Bleo formats offer stronger promoter activation and