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We confirmed these qualitative observations by performing a manual quantification within the hypothalamus (Fig

The protein was diluted in 20 mM HEPES, 150 mM NaCl, 10% glycerol to a final concentration of 0.2 mg ml 1

In general, plants can prevent chromium from entering the cell through external rejection, such as organic acids, amino acids, and proteins that combine with heavy metals (Chapman, Figure 2)

Uppuluri P, Lin L, Alqarihi A, Luo G, Youssef EG, Alkhazraji S, et al

Fourteen genes whose expression is significantly correlated with regional emphysema severity or transcription factors that are highly connected to these genes in the relevance network were selected for quantitative RT-PCR validation in a subset of tissue cores from subjects with severe emphysema (see Additional file 1 for methods)

Radiology 1994;193:297306