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Data Integration and Analysis Comprehensive data integration across multiple cycles provides valuable insights into epithalon's long-term effects and optimal protocol parameters

Storage guidance from vendors follows standard peptide practices: store lyophilized Epithalon in a cool, dry place away from light

The selectivity of FOXO4-DRI depends on the differential expression of FOXO4 between senescent and non-senescent cells

Cycle Length: 20 consecutive days on, then 46 months off

The hAMY 3 R cells were cultured in growth medium consisting of DMEM (Gibco, #31966-021) supplemented with 10% v/v FBS (Gibco, #16140-071), 1% v/v Penicillin-Streptomycin (Gibco, #15140-122), 0.5 mg/mL Geneticin G418 (Gibco, #10131-027), 0.4 mg/mL Hygromycin (Invitrogen, #10687010) and 250 nM Methotrexate (Sigma, A6770)

Figure 1A, B, and C Effect of Dihexa on spatial learning in the water maze