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Moreover, binding of nicotine to 7nAChR reduces the secretion of proinflammatory cytokines (TNF-, IL-1, IL6, and IL18) from LPS-stimulated human monocytic cell line and diminishes paralysis in EAE [233]

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Cells were passaged at a ratio of 1:3 every 2 days, and, to induce senescence, were treated with 8% CSE for 0, 2, 4, or 8 generations